Structure elucidation for tunable hetero-assembly of a plant PDX1.2/PDX1.3 pseudoenzyme-enzyme pair

ABSTRACT: Mass spectrometry raw data including peptide mapping, and top down liquid chromatography mass spectrometry for Arabidopsis PDX1.2/PDX1.3 homo- and hetero-complexes produced using cell-free expression. Purified proteins from cell-free expression were digested using trifluoroethanol protocol for peptide mapping. Proteins were directly analyzed for top down without additional treatment. Peptide data were analyzed using MS-GF+ and Byonic. The Byonic results were reported in the associated manuscript. Top down data were processed with ProMex for intact mass deconvolution.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive Plus

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Mowei Zhou  

PROVIDER: MSV000085233 | MassIVE | Mon Apr 06 08:29:00 BST 2020

REPOSITORIES: MassIVE