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PomGnt1 deficiency affects N-cadherin mediated cell-cell adhesion

ABSTRACT: N-glycoproteomic and O-man glycoproteomic analysis of N-cadherin derived from wild-type(WT) and POMGnT1-deficient HEK293T cells. Proteolytic digestion was performed with trypsin or proteinase K. Glycopeptide enrichment was achieved using cotton-HILIC SPE. Glycopeptides were fragmented by HCD.step and HCD.low. For O-man glycoproteomics N-glycan release by PNGase F was included.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Human

SUBMITTER: Marcus Hoffmann

PROVIDER: MSV000085243 | MassIVE | Tue Apr 07 08:58:00 BST 2020

REPOSITORIES: MassIVE

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