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PEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate

ABSTRACT: Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.

INSTRUMENT(S): NHMFL 21T FT-ICR-MS

ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145)

SUBMITTER: Lissa C. Anderson

PROVIDER: MSV000085258 | MassIVE | Wed Apr 08 18:02:00 BST 2020

REPOSITORIES: MassIVE

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PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry.

Takemori Ayako A Butcher David S DS Harman Victoria M VM Brownridge Philip P Shima Keisuke K Higo Daisuke D Ishizaki Jun J Hasegawa Hitoshi H Suzuki Junpei J Yamashita Masakatsu M Loo Joseph A JA Loo Rachel R Ogorzalek RRO Beynon Robert J RJ Anderson Lissa C LC Takemori Nobuaki N

Journal of proteome research 20200711 9

Prefractionation of complex mixtures of proteins derived from biological samples is indispensable for proteome analysis via top-down mass spectrometry (MS). Polyacrylamide gel electrophoresis (PAGE), which enables high-resolution protein separation based on molecular size, is a widely used technique in biochemical experiments and has the potential to be useful in sample fractionation for top-down MS analysis. However, the lack of a means to efficiently recover the separated proteins in-gel has a ...[more]

PMID: 32538093

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