Reactive Oxygen Species Oxidize STING and Suppress Interferon Production

ABSTRACT: No.1 Vehicle control. Bone marrow derived macrophages were treated with vehicle control for 30 mins. Cells were then lysed and alkylated by IAM, followed by immunoprecipitation of STING protein and gel separation of the protein. After digestion and secondary alkylation by NEM, sample was submitted for MS analysis. No.2 Menadione treated sample. Bone marrow derived macrophages were treated with Menadione for 30 mins. Cells were then lysed and alkylated by IAM, followed by immunoprecipitation of STING protein and gel separation of the protein. After digestion and secondary alkylation by NEM, sample was submitted for MS analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Tiffany Reese  

PROVIDER: MSV000085403 | MassIVE | Tue May 12 12:38:00 BST 2020

REPOSITORIES: MassIVE