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A comparison of Protein Extraction Methods in Aedes aegypti for Proteome Analysis

ABSTRACT: This study reports the identification of mosquito whole-body protein extracted using two methods: a conventional approach based on TCA/Acetone precipitation by following a method by Wang et al., (2015) and a commercial method based on formulated protein extraction reagent Cytobuster (Sigma, Germany). The resultant protein fragment was analyzed using QTOF ESI LCMS/MS.

INSTRUMENT(S): 1200 series LC/MSD SL

ORGANISM(S): Aedes Aegypti (ncbitaxon:7159)

SUBMITTER: Dr. Nurulhasanah Othman

PROVIDER: MSV000085571 | MassIVE | Wed Jun 10 07:53:00 BST 2020

SECONDARY ACCESSION(S): PXD019698

REPOSITORIES: MassIVE

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