Proteomics

Dataset Information

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Blood to plasma processing : multi-donor study of the effects of processing delay, temperature and anticoagulant type


ABSTRACT: This dataset contains raw mass spectrometry data (in the form of Thermo Fisher .RAW files) supporting associated publication “Variable blood processing procedures contribute to plasma proteomic variability” by Halvey et al. The experiments characterized by these data evaluated effects on human plasma proteome of the following factors involved in blood to plasma processing: delay to the first centrifugation, temperature and type of anticoagulant tube. Each combination of these factors has been applied to samples from n=4 healthy volunteers. Sample annotation provided in the file “Blood_to_Plasma_Processing_Sample_Annotation.txt” maps each sample to combination of those factors and anonymous subject identifier. For “Experiment5” there were several instances of LC-MS/MS analytical replicates for this sample. It should be noted that for this experiment the .RAW file from the “./updates/2020-08-03_vfarutin_e264fbfc/raw/” folder under this dataset FTP address represents the actual LC-MS/MS run used to generate the data in the associated paper by Halvey et al.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anthony M. Manning  

PROVIDER: MSV000085814 | MassIVE | Thu Jul 23 09:29:00 BST 2020

REPOSITORIES: MassIVE

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Publications

Variable blood processing procedures contribute to plasma proteomic variability.

Halvey Patrick P   Farutin Victor V   Koppes Laura L   Gunay Nur Sibel NS   Pappas Dimitrios A DA   Manning Anthony M AM   Capila Ishan I  

Clinical proteomics 20210119 1


<h4>Background</h4>Plasma is a potentially rich source of protein biomarkers for disease progression and drug response. Large multi-center studies are often carried out to increase the number of samples analyzed in a given study. This may increase the chances of variation in blood processing and handling, leading to altered proteomic results. This study evaluates the impact of blood processing variation on LC-MS/MS proteomic analysis of plasma.<h4>Methods</h4>Initially two batches of patient pla  ...[more]

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