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Astrocyte SplitBioID LFQ Proteomics

ABSTRACT: LC-MS/MS analysis of Split-BirA expressed in mouse Astrocytes. Data aquired on a Fusion Lumos and subjected to label-free relative quantitation withing Protein Discoverer (v 4.2). Sample key; Split-TuboID (47826, 47827, 47825) soluble TurboID (47838, 47839, 47840) TurboID-surface (TurboID-GPI; 47833, 47834, 47835)

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Scott Soderling

PROVIDER: MSV000085821 | MassIVE | Thu Jul 23 14:04:00 BST 2020

REPOSITORIES: MassIVE

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Chemico-genetic discovery of astrocytic control of inhibition in vivo.

Takano Tetsuya T Wallace John T JT Baldwin Katherine T KT Purkey Alicia M AM Uezu Akiyoshi A Courtland Jamie L JL Soderblom Erik J EJ Shimogori Tomomi T Maness Patricia F PF Eroglu Cagla C Soderling Scott H SH

Nature 20201111 7837

Perisynaptic astrocytic processes are an integral part of central nervous system synapses<sup>1,2</sup>; however, the molecular mechanisms that govern astrocyte-synapse adhesions and how astrocyte contacts control synapse formation and function are largely unknown. Here we use an in vivo chemico-genetic approach that applies a cell-surface fragment complementation strategy, Split-TurboID, and identify a proteome that is enriched at astrocyte-neuron junctions in vivo, which includes neuronal cell ...[more]

PMID: 33177716

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