The Capture of a Disabled Proteasome Identifies Erg25 as a Substrate for Endoplasmic Reticulum Associated Degradation

ABSTRACT: Proteasome, proteasome partners and ubiquitin-proteasome system (UPS) substrates were affinity purified from a yeast strain with an GFP-tagged proteasome subunit to which a proteasome inhibitor could be applied. Parallel experiments utilized inhibitor insensitive strains or strains lacking the tagged subunit. After affinity isolation, enriched proteins were resolved in SDS-PAGE, in-gel digested, and analyzed by high resolution liquid chromatography-tandem MS.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae By4741 (ncbitaxon:1247190)

SUBMITTER: Jeffrey L. Brodsky  

PROVIDER: MSV000085914 | MassIVE | Wed Aug 05 07:21:00 BST 2020

SECONDARY ACCESSION(S): PXD020736

REPOSITORIES: MassIVE