Proteomics

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Ion mobility fractionation enhanced mass spectrometry acquisition method for single-cell proteomics

ABSTRACT: Development and evaluation of the TIFF (Transferring Identification based on FAIMS Fractionation) method by benchmarking with standard methods. Single HeLa cells, LPS-treated RAW cells, and non-depleted human lung dissociated single cells were analyzed as applications of the TIFF method for single cell proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Ying Zhu  

PROVIDER: MSV000085937 | MassIVE | Mon Aug 10 12:54:00 BST 2020

REPOSITORIES: MassIVE

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Publications

Three-dimensional feature matching improves coverage for single-cell proteomics based on ion mobility filtering.

Woo Jongmin J   Clair Geremy C GC   Williams Sarah M SM   Feng Song S   Tsai Chia-Feng CF   Moore Ronald J RJ   Chrisler William B WB   Smith Richard D RD   Kelly Ryan T RT   Paša-Tolić Ljiljana L   Ansong Charles C   Zhu Ying Y  

Cell systems 20220316 5

Single-cell proteomics (scProteomics) promises to advance our understanding of cell functions within complex biological systems. However, a major challenge of current methods is their inability to identify and provide accurate quantitative information for low-abundance proteins. Herein, we describe an ion-mobility-enhanced mass spectrometry acquisition and peptide identification method, transferring identification based on FAIMS filtering (TIFF), to improve the sensitivity and accuracy of label-  ...[more]

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