Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
Project description:During discovery and development of protein-based drugs, extensive animal testing is required for preclinical analysis of pharmacokinetics, tissue biodistribution, toxicity, etc. Generally, several animals are required for testing a single drug. We show that the use of genetically encoded peptide barcodes, called flycodes, allows for the simultaneous preclinical assessment of dozens of antibodies from single cassette-dosed mice. Datasets: 1. comparison of flycodes vs. unique endogenous tryptic peptides for antibody identification 2. antibody PK, cassette dose A (high dose) 3. antibody PK, cassette dose B (low dose) 4. antibody tumor/organ biodistribution 5. Dar-SB thermostability 6. Dar-SB PK
2025-02-26 | PXD036662 | Pride
Project description:human single-chain variable fragment antibody against CXCR4
| PRJEB35859 | ENA
Project description:Transgenic hookworm secretes anti-tetrodotoxin human single chain antibody
Project description:<p>We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in healthy adults in two subsequent years. We determined vaccine response patterns specific to LAIV and TIV and found antibody sequences that were shared between two samples of the same individuals following influenza vaccination in subsequent years, thereby providing a genetic measurement of B-cell memory recall.</p>