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Moisture Modulates Soil Reservoirs of Active DNA and RNA viruses

ABSTRACT: Global proteomics analyses to identify viral proteins in the soil microbiome. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Environmental Samples <bacteria> (ncbitaxon:48479)

SUBMITTER: Kristin E. Burnum-Johnson

PROVIDER: MSV000086144 | MassIVE |

REPOSITORIES: MassIVE

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Moisture modulates soil reservoirs of active DNA and RNA viruses.

Wu Ruonan R Davison Michelle R MR Gao Yuqian Y Nicora Carrie D CD Mcdermott Jason E JE Burnum-Johnson Kristin E KE Hofmockel Kirsten S KS Jansson Janet K JK

Communications biology 20210826 1

Soil is known to harbor viruses, but the majority are uncharacterized and their responses to environmental changes are unknown. Here, we used a multi-omics approach (metagenomics, metatranscriptomics and metaproteomics) to detect active DNA viruses and RNA viruses in a native prairie soil and to determine their responses to extremes in soil moisture. The majority of transcribed DNA viruses were bacteriophage, but some were assigned to eukaryotic hosts, mainly insects. We also demonstrated that h ...[more]

PMID: 34446837

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Moisture modulates soil reservoirs of active DNA and RNA viruses.

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