A Chemical Acetylation based Mass Spectrometry Platform for Histone Profiling

ABSTRACT: Here we present a strategy for the identification and quantitation of histone methylation that utilizes nondeuterated acetic anhydride to fully acetylate all free lysine residues prior to digestion with trypsin and analysis by LC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Roland S. Annan  

PROVIDER: MSV000086146 | MassIVE |

REPOSITORIES: MassIVE