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Identification by Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry of Bacteria Cultured from the Microbiome of Maize

ABSTRACT: n this study, we optimized a bioinformatics pipeline to dereplicate bacteria isolated from the rhizosphere and endosphere of maize based on cluster analysis of protein spectra generated by MALDI-TOF. Readily-culturable bacteria were isolated from two varieties of maize (Zea mays): an agronomically important hybrid and a heritage. Isolates were sorted, following multiple iterations of alignment attempts, into clusters based on similarity of protein spectra generated by MALDI-TOF.

INSTRUMENT(S): ultraflex

ORGANISM(S): Mixed Culture Bacterium

SUBMITTER: Michael G. LaMontagne

PROVIDER: MSV000086274 | MassIVE | Mon Oct 12 16:58:00 BST 2020

REPOSITORIES: MassIVE

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