Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry

ABSTRACT: We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a <1.0% false discovery rate at the peptide and protein levels, we identified 3,731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2,000 additional single amino acid variants.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jong Shin Yoo  

PROVIDER: MSV000086444 | MassIVE | Thu Nov 12 08:06:00 GMT 2020

SECONDARY ACCESSION(S): PXD010025

REPOSITORIES: MassIVE