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Towards predicting the fate of reef corals

ABSTRACT: An iTRAQ approach was used to characterize the proteomes of reef corals (Orbicella faveolata) and their dinoflagellate endosymbionts (family Symbiodiniaceae) from a short-term (one month) thermal stress experiment. Please note that the same protein sample was labeled with the 113 tag and run in each batch (A, B, and C in the file names) to serve as an internal control aimed at limiting bias associated with batch-to-batch variation. As such, although 24 samples were analyzed (8-plex iTRAQ was used), only 21 of these reflect actual experimental coral samples. Please see the attached Word and Excel files to match the batch and tag with the experimental samples. Note that one sample (B5-7; iTRAQ batch A-label 114; a control coral sampled after five treatment days) spilled in the speed-vac during preparation and so was not analyzed, resulting in a final sample size of 20.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Orbicella Faveolata (ncbitaxon:48498) Breviolum Sp. (ncbitaxon:2499526) Durusdinium Sp. Clade D (ncbitaxon:298137)

SUBMITTER: Anderson Mayfield

PROVIDER: MSV000086530 | MassIVE | Fri Nov 27 07:54:00 GMT 2020

SECONDARY ACCESSION(S): PXD022796

REPOSITORIES: MassIVE

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Project description:Time course of batch growth. Reference (channel 1) was a culture grown in MD medium with 2.4 g/L glucose, with 5 slpm air-flow, stirring at 400 rpm and a constant 300C temperature, dilution 0.25 volumes/hour. The experimental (channel 2) time course samples were grown in batch for the indicated time. The "Low-D chemostat vs. High-D chemostat" hybridization's channel 2 sample was grown in a chemostat, with a dilution rate of 0.05 volumes/hour; it is most similar to the time course samples taken between 8 and 9 hours. Groups of assays that are related as part of a time series. FactorCategory: Elapsed Time; name: Elapsed Time; Measurement: time(absolute) 0 h; name: 45181_Elapsed Time; Measurement: time(absolute) 8.5 h; name: 38150_Elapsed Time; Measurement: time(absolute) 8.75 h; name: 38151_Elapsed Time; Measurement: time(absolute) 9 h; name: 38152_Elapsed Time; Measurement: time(absolute) 9.25 h; name: 38153_Elapsed Time; Measurement: time(absolute) 7.25 h; name: 38145_Elapsed Time; Measurement: time(absolute) 9.5 h; name: 38154_Elapsed Time; Measurement: time(absolute) 7.5 h; name: 38146_Elapsed Time; Measurement: time(absolute) 9.75 h; name: 38155_Elapsed Time; Measurement: time(absolute) 7.75 h; name: 38147_Elapsed Time; Measurement: time(absolute) 10 h; name: 38156_Elapsed Time; Measurement: time(absolute) 8 h; name: 38148_Elapsed Time; Measurement: time(absolute) 8.25 h; name: 38149_Elapsed Time FactorCategory: Growth Condition; name: Growth Condition; Growth Condition: chemostat dilution control; name: 45181_Growth Condition; Growth Condition: batch; name: 38150_Growth Condition; Growth Condition: batch; name: 38151_Growth Condition; Growth Condition: batch; name: 38152_Growth Condition; Growth Condition: batch; name: 38153_Growth Condition; Growth Condition: batch; name: 38145_Growth Condition; Growth Condition: batch; name: 38154_Growth Condition; Growth Condition: batch; name: 38146_Growth Condition; Growth Condition: batch; name: 38155_Growth Condition; Growth Condition: batch; name: 38147_Growth Condition; Growth Condition: batch; name: 38156_Growth Condition; Growth Condition: batch; name: 38148_Growth Condition; Growth Condition: batch; name: 38149_Growth Condition Keywords: time_series_design

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Towards predicting the fate of reef corals

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