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Identification/quantitation of human Cystatin C fragments

ABSTRACT: The data uploaded is related to the identification/quantitation of Cystatin C fragment 95-146 in human hemofiltrate. Also, the mass spectrometry analysis of Cystatin C digested with several proteases is included.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Human

SUBMITTER: Dr. Sebastian Wiese

PROVIDER: MSV000086609 | MassIVE | Wed Dec 16 23:20:00 GMT 2020

REPOSITORIES: MassIVE

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Natural cystatin C fragments inhibit GPR15-mediated HIV and SIV infection without interfering with GPR15L signaling.

Hayn Manuel M Blötz Andrea A Rodríguez Armando A Vidal Solange S Preising Nico N Ständker Ludger L Wiese Sebastian S Stürzel Christina M CM Harms Mirja M Gross Rüdiger R Jung Christoph C Kiene Miriam M Jacob Timo T Pöhlmann Stefan S Forssmann Wolf-Georg WG Münch Jan J Sparrer Konstantin M J KMJ Seuwen Klaus K Hahn Beatrice H BH Kirchhoff Frank F

Proceedings of the National Academy of Sciences of the United States of America 20210101 3

GPR15 is a G protein-coupled receptor (GPCR) proposed to play a role in mucosal immunity that also serves as a major entry cofactor for HIV-2 and simian immunodeficiency virus (SIV). To discover novel endogenous GPR15 ligands, we screened a hemofiltrate (HF)-derived peptide library for inhibitors of GPR15-mediated SIV infection. Our approach identified a C-terminal fragment of cystatin C (CysC95-146) that specifically inhibits GPR15-dependent HIV-1, HIV-2, and SIV infection. In contrast, GPR15L, ...[more]

PMID: 33431697

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