Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Profile of Rabbit Vocal Fold Tissue Systemically Dehydrated with Furosemide

ABSTRACT: The objective of this study was to obtain a comprehensive proteome of euhydrated and systemically dehydrated rabbit vocal folds. We hypothesized that a mild-moderate systemic dehydration level would lead to changes in protein levels. New Zealand White rabbits were randomly assigned to euhydrated (control) or furosemide-dehydrated groups. Systemic dehydration was assessed by body weight loss and changes in blood markers. Vocal fold specimens were processed for proteomic analysis using liquid chromatography-tandem mass spectrometry. Systemic dehydration induced 5.5 percent body weight loss (range: 4.5 to 6.5 percent) and significant changes in blood analytes compared to controls. More than 1500 proteins were identified across all vocal fold samples, associated with a variety of cellular components and ubiquitous cell functions such as protein synthesis and degradation, and mitochondrial translation and respiration. The comparison analysis identified 27 proteins differentially expressed in the systemically dehydrated vocal folds. These proteins are mainly involved with mitochondrial translation and metabolism, which are downregulated in response to systemic dehydration. We identified a large group of proteins representing the rabbit vocal fold tissue. The data provide novel insights on the response of vocal folds to a mild-moderate level of systemic dehydration that involves the downregulation of mitochondrial metabolism, suggesting a biologic mechanism to prevent oxidative damage associated with furosemide-induced dehydration.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Oryctolagus Cuniculus

SUBMITTER: M. Preeti Sivasankar Abigail Cox

PROVIDER: MSV000086612 | MassIVE | Thu Dec 17 08:45:00 GMT 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Vocal folds of older rabbits are more vulnerable to protein changes caused by systemic dehydration

Project description:Older subjects are more prone to develop systemic dehydration. Systemic dehydration has implications for vocal fold biology by affecting gene and protein expression. The interaction of hydration status and age has not been investigated in the context of vocal fold biology. We used comparative proteomics to analyze the vocal fold proteome of 6.5-month-old and over 3-year-old rabbits under water ad libitum or water volume restriction protocol. Young and old rabbits (n= 22) were either euhydrated (water ad libitum) or dehydrated by water volume restriction. Dehydration was confirmed by body weight loss of -5.4% and -4.6% in young and old groups, respectively, and a 1.7-fold increase of kidney renin gene expression in the young rabbits. LC-MS/MS identified 2,286 proteins in the rabbit vocal folds of young and old rabbits combined. Of these, 177, 169, and 81 proteins were significantlyaffected by age, hydration status, or the interaction of both factors, respectively. Comparative proteomics revealed a higher number of proteins differentially regulated in the vocal folds of older dehydrated rabbits compared to older euhydrated, and when compared to younger rabbits, regardless of hydration status. These proteins are predominantly related to structural components of the extracellular matrix and muscle layer, suggesting a disturbance in the viscoelastic properties of aging vocal fold tissue, especially when subjected to systemic dehydration.

2022-06-17 | MSV000089683 | MassIVE

Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model

Project description:The purpose of this study is to investigate if acute systemic dehydration impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered hydration state in response to furosemide on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. Together with cellular, structural, and functional techniques that are part of this project, we expect to provide validated scientific recommendations on the adverse effects of dehydration and the therapeutic benefits of hydration.

2020-07-09 | GSE132765 | GEO

Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model

Project description:Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model

| PRJNA548941 | ENA

Rattus norvegicus

Project description:Transcription in wounded rat vocal folds and vocal fold fibroblasts

| PRJNA263474 | ENA

RNA-Seq of vocal folds of rabbits exposed to a low relative humidity environment

Project description:The purpose of this study is to investigate if acute mucosal dehydration as a funcition of low relative humidty environment impacts the vocal folds at the mRNA level. Rabbits were used as animal model to enable a systematic and rigorous assessment of the pathobiology of vocal fold hydration since they can be manipulated in a physiologically realistic manner. We anticipate that the results of this study will help to elucidate the effects of altered mucosal hydration state in response to low ambient humidity on vocal fold epithelium, lamina propria and muscle tissue at the transcriptome level. Together with cellular, structural, and functional techniques that are part of this project, we expect to provide validated scientific recommendations on the adverse effects of mucosal dehydration and the therapeutic benefits of hydration.

2020-12-21 | GSE148588 | GEO

Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

Project description:Voice disorders are an important human health condition. Hydration is a commonly recommended preventive measure for voice disorders though it is unclear how vocal fold dehydration, is harmful at the cellular level. Airway surface dehydration can result from exposure to low humidity air. Here we have used a recurring 8-hour low humidity exposure over 15 days to mimic an occupational exposure to a low humidity environment. Exposure to moderate humidity was the control condition. Full thickness soft-tissue samples, including the vocal folds and surrounding laryngeal tissue, were collected for molecular analysis. RT-qPCR demonstrated a significant upregulation of MUC4 (mucin 4) and SCL26A9 (chloride channel) and a large fold-change though statistically non-significant upregulation of SCNNA1 (epithelial sodium channel). Proteomic analysis demonstrated differential regulation of proteins clustering into prospective functional groups of muscle structure and function, oxidative stress response, and the protein chaperonin stress response. Together, the data demonstrate that recurring exposure to low humidity is sufficient to induce both transcriptional and translational level changes in laryngeal tissue and suggest that low humidity exposure induces cellular stress at the level of the vocal folds.

2022-03-29 | MSV000089151 | MassIVE

Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

2020-12-07 | MSV000086568 | MassIVE

Expression data from rat vocal fold at different periods after vocal fold injury

Project description:Expression data from rat vocal fold at different periods after vocal fold injury

| PRJNA579528 | ENA

Proteomic analysis of vocal fold fibroblasts exposed to cigarette smoke extract

Project description:Reinke’s edema is a cigarette associated, benign, mostly bilateral lesion of the vocal folds leading to dysphonia and dyspnea. Until today information about pathophysiology is only scarce, treatment is only surgical. To explore the pathophysiology of Reinke’s edema, we exposed near primary human vocal fold fibroblasts to cigarette smoke extract enriched medium or air bubbled control for 24 hrs. followed by a proteomic analysis. Proteomic analyses revealed an increase of proteins involved in oxidative stress response. We furthermore found that a significant number of essential extracellular matrix proteins was altered by culturing human VFF with CSE enriched medium: UDP-glucose 6-dehydrogenase, a protein involved in hyaluronan synthesis was upregulated, while the expression of several fibrillar collagen types was significantly reduced. The current findings corroborate previous studies but revealed new insights in possible disease mechanisms of Reinke’s edema too. We postulate that changes in the composition -reduction of collagen fibrils, increase of hyaluronan- of the vocal folds’ ECM may lead to the clinical findings.

2019-08-06 | PXD010640 | Pride

Comparative Proteomics of PairedVocal Fold and Oral Mucosa Fibroblasts

Project description:Injuries of the vocal folds frequently heal with scar formation, which can have lifelong detrimental impact on voice quality. Current treatments to prevent or resolve scars of the vocal fold mucosa are highly unsatisfactory. In contrast, the adjacent oral mucosa is mostly resistant to scarring. These differences in healing tendency might relate to distinct properties of the fibroblasts populating oral and vocal fold mucosae. We thus established the in vitro cultivation of paired, near-primary vocal fold fibroblasts (VFF) and oral mucosa fibroblasts (OMF) to perform a basic cellular characterization and comparative cellular proteomics. VFF were significantly larger than OMF, proliferated more slowly, and exhibited a sustained TGF-β1-induced elevation of pro-fibrotic interleukin 6. Cluster analysis of the proteomic data revealed distinct protein repertoires specific for VFF and OMF. Further, VFF displayed a broader protein spectrum, particularly a more sophisticated array of factors constituting and modifying the extracellular matrix. Conversely, subsets of OMF-enriched proteins were linked to cellular proliferation, nuclear events, and protection against oxidative stress. Altogether, this study supports the notion that fibroblasts sensitively adapt to the functional peculiarities of their respective anatomical location and presents several molecular targets for further investigation in the context of vocal fold wound healing.

2017-01-23 | PXD005106 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data