Proteomics

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Experimental validation of a central carbon metabolic model in Aspergillus niger to enable metabolic engineering of a fungal cell factory

ABSTRACT: The influence of selected pentose catabolic pathway (PCP) deletion strains on growth of plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of the fungus Aspergillus niger in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains grown on wheat bran (WB) and sugar beet pulp (SBP) were evaluated. Proteomics samples were digested with trypsin then analyzed by LC-MS/MS with fractionation. Metabolomics samples were derivitized using a modified Fiehn protocol and analyzed by GC-MS with FAME added for retention alignment. Proteomics data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Agilent 7890A GC coupled to a 5975C inert XL MSD, Q Exactive Plus

ORGANISM(S): Aspergillus Niger (ncbitaxon:5061)

SUBMITTER: Ronald de Vries  

PROVIDER: MSV000086614 | MassIVE | Thu Dec 17 16:02:00 GMT 2020

SECONDARY ACCESSION(S): PXD023205

REPOSITORIES: MassIVE

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Publications

Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates.

Chroumpi Tania T   Peng Mao M   Markillie Lye Meng LM   Mitchell Hugh D HD   Nicora Carrie D CD   Hutchinson Chelsea M CM   Paurus Vanessa V   Tolic Nikola N   Clendinen Chaevien S CS   Orr Galya G   Baker Scott E SE   Mäkelä Miia R MR   de Vries Ronald P RP  

Frontiers in bioengineering and biotechnology 20210308

The filamentous ascomycete <i>Aspergillus niger</i> has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected  ...[more]

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