Proteomics

Dataset Information

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Phosphoproteomic Response of Cardiac Endothelial Cells to Ischemic Injury and Ultrasound

ABSTRACT: Global and phosphoproteomic data from mouse primary cardiac endothelial cells treated with oxygen deprivation and/or ultrasound. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Jon Jacobs  

PROVIDER: MSV000086652 | MassIVE | Thu Dec 31 12:50:00 GMT 2020

SECONDARY ACCESSION(S): PXD023331

REPOSITORIES: MassIVE

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Publications

(Phospho)Proteomic dataset of ischemia- and ultrasound- stimulated mouse cardiac endothelial cells in vitro.

Emechebe Uchenna U   Giraud David D   Ammi Azzdine Y AY   Scott Kristin L KL   Jacobs Jon M JM   McDermott Jason E JE   Dykan Igor V IV   Alkayed Nabil J NJ   Barnes Anthony P AP   Kaul Sanjiv S   Davis Catherine M CM  

Data in brief 20210904

Cardiac endothelial cells respond to both ischemia and therapeutic ultrasound; the proteomic changes underlying these responses are unknown. This data article provides raw and processed data resulting from our global, unbiased phosphoproteomics investigation conducted on primary mouse cardiac endothelial cells exposed to ischemia (2-hour oxygen glucose deprivation) and ultrasound (250 kHz, 1.2 MPa) in vitro [1]. Proteins were extracted from cell lysates and enriched phosphopeptides were analyzed  ...[more]

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