Dataset Information

Top-down proteomic analysis with FAIMS of Alzheimer's disease derived brain tissue

ABSTRACT: Proteomic datasets collected from a single human midfrontal cortex tissue sample. Tissue was homogenized in 8 M Urea, 10 mM ABC, and 10 mM TCEP before subsequent cleanup with 100K and 3K MWCO filters. NanoLC was performed for RP separation. Three technical replicates were collected without FAIMS. Seven FAIMS compensation voltages with three technical replicates each were also collected, for a total of 21 FAIMS datasets. Raw files were collected in "full-profile" mode on a Thermo Fusion Lumos mass spectrometer. Data was searched with TopPIC v1.3 using PNNL's DMS processing pipeline.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Vladislav A. Petyuk

PROVIDER: MSV000086696 | MassIVE | Thu Jan 14 15:26:00 GMT 2021

SECONDARY ACCESSION(S): PXD023607

REPOSITORIES: MassIVE

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Enhancing Top-Down Proteomics of Brain Tissue with FAIMS.

Fulcher James M JM Makaju Aman A Moore Ronald J RJ Zhou Mowei M Bennett David A DA De Jager Philip L PL Qian Wei-Jun WJ Paša-Tolić Ljiljana L Petyuk Vladislav A VA

Journal of proteome research 20210415 5

Proteomic investigations of Alzheimer's and Parkinson's disease have provided valuable insights into neurodegenerative disorders. Thus far, these investigations have largely been restricted to bottom-up approaches, hindering the degree to which one can characterize a protein's "intact" state. Top-down proteomics (TDP) overcomes this limitation; however, it is typically limited to observing only the most abundant proteoforms and of a relatively small size. Therefore, fractionation techniques are ...[more]

PMID: 33856812

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