Top-down proteomic analysis with FAIMS of Alzheimer's disease derived brain tissue

ABSTRACT: Proteomic datasets collected from a single human midfrontal cortex tissue sample. Tissue was homogenized in 8 M Urea, 10 mM ABC, and 10 mM TCEP before subsequent cleanup with 100K and 3K MWCO filters. NanoLC was performed for RP separation. Three technical replicates were collected without FAIMS. Seven FAIMS compensation voltages with three technical replicates each were also collected, for a total of 21 FAIMS datasets. Raw files were collected in "full-profile" mode on a Thermo Fusion Lumos mass spectrometer. Data was searched with TopPIC v1.3 using PNNL's DMS processing pipeline.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Vladislav A. Petyuk  

PROVIDER: MSV000086696 | MassIVE | Thu Jan 14 15:26:00 GMT 2021

SECONDARY ACCESSION(S): PXD023607

REPOSITORIES: MassIVE