Proteomics

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Resource allocation in the archaeon Methanococcus maripaludis during slow growth


ABSTRACT: We conducted a systems-level study on the physiology of slow growth in the archaeon Methanococcus maripaludis revealing a strategy for acclimation to slow growth that relies on redistribution of energy for cellular maintenance and changes in catabolic and ribosomal activity rather than gene regulation, a strategy that is distinct from well-known heterotrophic model bacteria such as E. coli. This dataset describes the proteome response of Methanococcus maripaludis to a formate (energy) limitation when grown in a chemostat at different growth rates. Samples contain both 14N and 15N-labeled peptides, with the 15N reference generated as a combination of batch exponential culture and a stationary phase cultures.

INSTRUMENT(S): TripleTOF 5600

SUBMITTER: Alfred M. Spormann   James R. Williamson  

PROVIDER: MSV000086844 | MassIVE | Thu Feb 11 14:22:00 GMT 2021

SECONDARY ACCESSION(S): PXD024262

REPOSITORIES: MassIVE

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Publications

An alternative resource allocation strategy in the chemolithoautotrophic archaeon Methanococcus maripaludis.

Müller Albert L AL   Gu Wenyu W   Patsalo Vadim V   Deutzmann Jörg S JS   Williamson James R JR   Spormann Alfred M AM  

Proceedings of the National Academy of Sciences of the United States of America 20210401 16


Most microorganisms in nature spend the majority of time in a state of slow or zero growth and slow metabolism under limited energy or nutrient flux rather than growing at maximum rates. Yet, most of our knowledge has been derived from studies on fast-growing bacteria. Here, we systematically characterized the physiology of the methanogenic archaeon Methanococcus maripaludis during slow growth. M. maripaludis was grown in continuous culture under energy (formate)-limiting conditions at different  ...[more]

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