Proteomics

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Halo-Control/WDR76/SPIN1/CBX5_purification_MudPIT_wPTMs

ABSTRACT: Halo purification samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are Halo-WDR76, Halo-SPIN1, Halo-CBX5. The bait for Control Purification is Halo tag only. Purification for each bait was performed using whole cell extract from a stable cell line in 293FRT cells. 3 biological replicates for each type of bait.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael P. Washburn  

PROVIDER: MSV000086846 | MassIVE | Thu Feb 11 15:21:00 GMT 2021

REPOSITORIES: MassIVE

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An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome.

Liu Xingyu X   Zhang Ying Y   Wen Zhihui Z   Hao Yan Y   Banks Charles A S CAS   Cesare Joseph J   Bhattacharya Saikat S   Arvindekar Shreyas S   Lange Jeffrey J JJ   Xie Yixuan Y   Garcia Benjamin A BA   Slaughter Brian D BD   Unruh Jay R JR   Viswanath Shruthi S   Florens Laurence L   Workman Jerry L JL   Washburn Michael P MP  

Proceedings of the National Academy of Sciences of the United States of America 20240808 33

Serial capture affinity purification (SCAP) is a powerful method to isolate a specific protein complex. When combined with cross-linking mass spectrometry and computational approaches, one can build an integrated structural model of the isolated complex. Here, we applied SCAP to dissect a subpopulation of WDR76 in complex with SPIN1, a histone reader that recognizes trimethylated histone H3 lysine4 (H3K4me3). In contrast to a previous SCAP analysis of the SPIN1:SPINDOC complex, histones and the  ...[more]

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