The impact of E2F/Dp inactivation on metabolism differs between muscles and fat body cells

ABSTRACT: We aim to determine the physiological impact of inactivating E2F in muscles and in adipose cells in vivo. To uncover the mechanism of action of E2F in each tissue we investigated the similarities between E2F-deficient tissues by comparing the proteomic profiles between muscles and fat cells. Our findings revealed that E2F is required in fat cells to promote the storage of fat and the synthesis of trehalose, which was restored by feeding animals in high sugar diet. Multiplexed quantitative proteomics was performed using TMT10-plex reagents on an Orbitrap Fusion mass spectrometer using the SPS-MS3 method. Two sample sets - fatbody samples and samples of thoracic muscles - were analyzed using two TMT sets. Twelve high pH reversed-phase chromatography fractions were analyzed per sample set. The samples in each set were TMT-labeled as follows: Fatbody samples: WT FB MS1; 126 DP FB MS 1; 127n WT FB MS2; 127c DP FB MS 2; 128n WT FB MS3; 128c DP FB MS 3; 129n Thoracic muscles samples: Mef>Ci 1; 126 Mef>Dpi 1; 127n Mef>Ci 2; 127c Mef>Dpi 2; 128n Mef>Ci 3; 128c Mef>Dpi 3; 129n Mef>Ci 4; 129c Mef>Dpi 4; 130n

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227)

SUBMITTER: Maxim V Frolov  

PROVIDER: MSV000086854 | MassIVE | Thu Feb 11 21:43:00 GMT 2021

REPOSITORIES: MassIVE