Klein / Husselmann chia under salt and caffeic acid stress

ABSTRACT: Husselmann 2017: Following gel and TOF/TOF investigation of chia salinity stress by Achmat Williams [http://hdl.handle.net/11394/5650], Husselmann and Klein designed an LC-MS/MS experiment for five replicates in each of five cohorts: caffeic acid stress, salt stress, both stresses, and controls for black chia seeds, along with controls for white chia seeds. Extracted proteins were analyzed at the Centre for Proteome and Genome Research. Bell denatured proteins, reduced disulfides with TCEP and alkylated cysteines with MMTS, and digested proteins with trypsin in a HILIC magnetic bead workflow. The RPLC gradients yielded an average of 34,512 tandem mass spectra per LC-MS/MS experiment on the Thermo Q Exactive. Samples pooling all cohorts were also run in quadruplicate using the same method for normalization.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Salvia Hispanica (ncbitaxon:49212)

SUBMITTER: Ashwil Klein  

PROVIDER: MSV000086861 | MassIVE | Fri Feb 12 13:59:00 GMT 2021

REPOSITORIES: MassIVE