Molecular physiological characterization of a high heat resistant spore forming Bacillus subtilis food isolate

ABSTRACT: Bacterial endospores (spores) are among the most resistant living forms on earth. Spores of Bacillus subtilis A163 show extremely high resistance to wet heat compared to spores of laboratory strains. In this study, we found that spores of B. subtilis A163 were indeed very wet heat resistant and released dipicolinic acid (DPA) very slowly during heat treatment. We also determined the proteome of vegetative cells and spores of B. subtilis A163 and the differences in these proteomes from those of the laboratory strain PY79, spores of which are much less heat resistant. This proteomic characterization identified 2011 proteins in spores and 1901 proteins in vegetative cells of B. subtilis A163.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Bacillus Subtilis

SUBMITTER: Gertjan Kramer  

PROVIDER: MSV000087080 | MassIVE | Sun Mar 21 13:29:00 GMT 2021

SECONDARY ACCESSION(S): PXD024885

REPOSITORIES: MassIVE