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Mass spectrometric analysis of fluorophore labeled albumins

ABSTRACT: A genetic engineering strategy for editing near-infrared-II fluorophores. The labeled albumin proteins were digested using suspension trapping (STrap) approach with in-house packed glass fiber filters. The LC-MS/MS analysis was performed on a nanoLC-Q Exactive MS system. The protein identification was performed using Proteome Discoverer (version 2.3) and MaxQuant (version 1.6.3.4).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: CHEN Xiaoyuan, Shawn

PROVIDER: MSV000087386 | MassIVE | Fri May 07 12:36:00 BST 2021

REPOSITORIES: MassIVE

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A genetic engineering strategy for editing near-infrared-II fluorophores.

Tian Rui R Feng Xin X Wei Long L Dai Daoguo D Ma Ying Y Pan Haifeng H Ge Shengxiang S Bai Lang L Ke Chaomin C Liu Yanlin Y Lang Lixin L Zhu Shoujun S Sun Haitao H Yu Yanbao Y Chen Xiaoyuan X

Nature communications 20220523 1

The second near-infrared (NIR-II) window is a fundamental modality for deep-tissue in vivo imaging. However, it is challenging to synthesize NIR-II probes with high quantum yields (QYs), good biocompatibility, satisfactory pharmacokinetics, and tunable biological properties. Conventional long-wavelength probes, such as inorganic probes (which often contain heavy metal atoms in their scaffolds) and organic dyes (which contain large π-conjugated groups), exhibit poor biosafety, low QYs, and/or unc ...[more]

PMID: 35606352

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