Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RajKumar-PolZeta-P19-078_MayoClinic

ABSTRACT: Silver stained bands were submitted for mass spec analysis. Investigators are interested in the protein polzeta.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Rajiv Kumar

PROVIDER: MSV000087408 | MassIVE | Wed May 12 07:41:00 BST 2021

SECONDARY ACCESSION(S): PXD025981

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

DrRajKumar-Rev1_P20-025_MayoClinic

Project description:Coomassie blue stained bands were submitted for mass spec analysis studying the protein Rev1.

2021-05-12 | MSV000087410 | MassIVE

PME1 interactome - Relevance Rank Platform (RRP) for Functional Filtering of High Content Protein-Protein Interaction Data

Project description:For a single step AP-MS identification of PME1 interactome was established a cell line stably expressing either Strep-tag alone or Strep_PME-1 fusion protein. After Strep-tag affinity purification, proteins were separated on SDS-PAGE gel, silver stained and protein bands that showed visually enrichment in StrepPME-1 samples compared to Strep-tag samples were isolated from both lanes for MS analysis.

2015-12-08 | PXD002637 | Pride

Immunoreactive proteins in the oesophageal gland cells of Anisakis simplex sensu stricto detected by MALDI-TOF/TOF analysis

Project description:Two hundred ventricles were manually dissected from L3 stage larvae of Aniskakis simplex s.s. to allow direct protein analysis. Denaturing gel electrophoresis followed by monochromatic silver staining revealed the presence of differential (enriched) proteins when compared to total nematode extracts. Such comparison was performed by means of 1D and 2D electrophoresis. Pooled antisera from Anisakis spp allergic patients were used in western blots revealing the presence of 13 immunoreactive bands in the ventricular extracts in 1D, while 82 spots in 2D. The corresponding protein bands and spots were excised from the silver stained gel and protein assignation was made by MALDI-TOF/TOF. A total of eleven proteins (including isoforms) were unambiguosly identified. The majority of these proteins are known to be secreted by nematodes into the external environment, of which three are described as being major allergens in other organisms with different phylogenetic origin.

2021-09-09 | PXD019580 | Pride

Identification of ADAR1 associated proteins

Project description:To understand how ADAR1 controls genome stability, affinity purification and mass spectrometry were employed to identify the interactomes of ADAR1 with cellular extracts from HeLa cells stably expressing FLAG-ADAR1.Mass spectrometry analysis of FLAG-ADAR1 associated proteins. The differential protein bands of the immunoprecipitates from cellular extracts of HeLa cells were retrieved from silver stained SDS-PAGE, digested with trypsin and analyzed by mass spectrometry.

2022-11-15 | PXD038167 | iProX

Identification of LGALS1 binding partners in T84 cells by proteomics using a photocrosslinking GlcNAc analog

Project description:Surface glycoproteins from T84 cells were metabolically incorporated with a diazirine functionalized GlcNAc analog and crosslinked to biotinylated LGALS1 on intact cells. The crosslinked glycoproteins were enriched by streptavidin beads and separated by SDS PAGE. Crosslinked bands identified by silver stain were analyzed by proteomics. Regions at the same molecular weight from the silver stained gel but from a sample that was not incorporated with the diazirine functionalized GlcNAc analog and not crosslinked to biotinylated LGALS1 were used as negative controls.

2021-06-16 | MSV000087640 | MassIVE

Mass spectrometry identification data for the interactomics of SMYD3

Project description:SMYD3, a member of the SET and MYND domain-containing (SMYD) family, is a histone methyltransferase (HMT) and transcription factor that plays an important role in transcriptional regulation in human carcinogenesis.In an effort to better understanding the mechanistic role of SMYD3, affinity purification and mass spectrometry assays were used to identify proteins associated with SMYD3 in vivo. In these experiments, FLAG-tagged SMYD3 or an empty vector was stably expressed in LM3 cells. Cellular extracts were prepared and subjected to affinity purification using an anti-FLAG affinity gel. Immunocomplexed proteins were separated using SDS–PAGE and silver stained. Immunoprecipitated proteins in specific bands in comparison to the vector were gel extracted, trypsin digested and identified using liquid chromatography tandem mass spectrometry.

2023-03-11 | PXD031971 | Pride

New protein hormone peptides were identified by mass spectrometry of mouse serum protein under western diet state.

Project description:We have discovered a protein hormone that is induced to secrete under western diet. We used SDS-PAGE and silver staining to compare the differential bands of all proteins in the serum of mice under the chow diet state and under the western diet state, and identified several peptides of this protein under the western diet state by mass spectrometry analysis.

2024-03-25 | PXD040153 | Pride

The interactome of G3BP1 in UMUC3 cells

Project description:The HA-tagged G3BP1 complex was obtained by co-IP with anti-HA antibodies from UMUC3 cells stably expressing HA-tagged G3BP1. The purified immunoprecipitants were resolved on gradient SDS-PAGE, silver-stained, and subjected to MS analysis.

2024-05-23 | PXD035722 | Pride

Mass spec results of Tmc7-GST pull-down

Project description:For the GST pull-down of DRG ganglion, the GST- or Tmc7-GST-associated beads were further incubated with cell lysates derived from DRG ganglion. The pulled-down proteins were then separated by SDS-PAGE gel electrophoresis and subsequently stained with silver staining Kit. Finally, the target molecular weight bands and undenatured beads were analyzed by proteomic analysis, respectively.

2024-03-08 | PXD050466 | iProX

Proteomic analysis of S-nitrosylation in HUVECs

Project description:HUVECs stimulated with HG+oxLDL or Mannitol+nLDL were subjected to the biotin-switch assay to enrich the S-nitrosylated proteins, the S-nitrosylated proteins were resolved by SDS-PAGE and gels were silver stained. Mass spectrometry was conducted to determine proteins with S-nitrosylation.

2021-06-03 | PXD025295 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data