An ultrasensitive method for analysis of viral spike N-glycoforms

ABSTRACT: Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike proteins as potential vaccines, this method is applicable for analysis of site-specific glycosylation of any glycoprotein.

INSTRUMENT(S): timsTOF Pro, Q Exactive HF-X

ORGANISM(S): Bg505 Sosip.664 Md39 Bg505 Sosip.664 Homo Sapiens (ncbitaxon:9606)

SUBMITTER: John R. Yates III  

PROVIDER: MSV000087414 | MassIVE | Wed May 12 20:28:00 BST 2021

SECONDARY ACCESSION(S): PXD025990

REPOSITORIES: MassIVE