Mass spectrometry-based direct detection of multiple types of protein thiol modifications in pancreatic beta cells under endoplasmic reticulum stress

ABSTRACT: A 2D-LC-MS/MS strategy coupled with TMT-10 labeling was applied to mouse pancreatic beta cells (hetaTC-6) induced with ER stress by Thapsigargin to study the global proteome and protein cysteine PTM changes under ER stress. Samples were digested with trypsin, labeled with 10-plex TMT, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Wei-Jun Qian  

PROVIDER: MSV000087543 | MassIVE | Sat May 29 20:39:00 BST 2021

SECONDARY ACCESSION(S): PXD026358

REPOSITORIES: MassIVE