Pseudomonas aeruginosa PA14 biofilms produce R-bodies, extendable protein polymers with roles in host colonization and virulence

ABSTRACT: Paper submitted to Nature Communications (2021): "Pseudomonas aeruginosa PA14 biofilms produce R-bodies, extendable protein polymers with roles in host colonization and virulence." Abstract: We used a modified R-body enrichment protocol to isolate SDS-insoluble fractions from PA14 biofilms and performed protein mass spectrometry (MS). We detected products of the reb cluster by MS. RebP1 and RebP2, which were detected in a ~1:1 ratio, were among the 12 most abundant proteins in our sample. List of Files: Q Exactive HF Delta pel1 LD1_123015_012016_98min_5uL_1.raw Delta pel2 LD2_123015_012016_98min_5uL_1.raw WT LD3_123015_98min_12uL_1_160120171453.raw Synapt G2 Delta pel1 LD1_123015_011316_5uL_1.raw LD1_123015_011316_5uL_2.raw Delta pel2 LD2_123015_011316_5uL_1.raw LD2_123015_011316_5uL_2.raw WT LD3_123015_011316_5uL_3.raw LD3_123015_011316_5uL_4.raw

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Pseudomonas Aeruginosa

SUBMITTER: Lars Dietrich   Lewis M. Brown  

PROVIDER: MSV000087657 | MassIVE | Fri Jun 18 09:17:00 BST 2021

REPOSITORIES: MassIVE