Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: An Chi
PROVIDER: MSV000087954 | MassIVE | Fri Aug 06 20:04:00 BST 2021
SECONDARY ACCESSION(S): PXD018573
REPOSITORIES: MassIVE
Ruprecht Benjamin B Di Bernardo Julie J Wang Zhao Z Mo Xuan X Ursu Oleg O Christopher Matthew M Fernandez Rafael B RB Zheng Li L Dill Brian D BD Wang Huijun H Xu Yuting Y Liaw Andy A Mortison Jonathan D JD Siriwardana Nirodhini N Andresen Brian B Glick Meir M Tata James R JR Kutilek Victoria V Cornella-Taracido Ivan I Chi An A
Nature chemical biology 20200720 10
Mass spectrometry-based discovery proteomics is an essential tool for the proximal readout of cellular drug action. Here, we apply a robust proteomic workflow to rapidly profile the proteomes of five lung cancer cell lines in response to more than 50 drugs. Integration of millions of quantitative protein-drug associations substantially improved the mechanism of action (MoA) deconvolution of single compounds. For example, MoA specificity increased after removal of proteins that frequently respond ...[more]