Proteomics

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Linking soil microbial community traits to the soil storage potential of dissolved organic carbon from surface plant litter


ABSTRACT: Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase plant litter degradation, we found that proteins and condensed hydrocarbons are the compounds with the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins and condensed hydrocarbons correlated negatively with DOC. With nuclear magnetic resonance (NMR) spectroscopy, we identified 15 individual compounds associated with DOC concentration. Through random forest, neural network, and indicator species analyses, we identified bacterial and fungal taxa associated with DOC concentration and additionally identified connections between microorganisms and DOC chemical composition.

INSTRUMENT(S): solariX

ORGANISM(S): Environmental Samples <bacteria> (ncbitaxon:48479)

SUBMITTER: John Dunbar  

PROVIDER: MSV000088109 | MassIVE |

REPOSITORIES: MassIVE

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Publications

Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition.

Campbell Tayte P TP   Ulrich Danielle E M DEM   Toyoda Jason J   Thompson Jaron J   Munsky Brian B   Albright Michaeline B N MBN   Bailey Vanessa L VL   Tfaily Malak M MM   Dunbar John J  

Frontiers in microbiology 20220120


Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential  ...[more]

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