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Discovery of Dehydroamino Acid Residues in the Capsid and Matrix Structural Proteins of HIV-1

ABSTRACT: Bottom-up mass spectrometry-based proteomic analysis (trypsin) was performed on four biological replicates of HIV-1 virions. These virions were isolated from HEK293T cells transfected with a HIV-1 proviral plasmid derived from the pNL4-3 molecular clone, rendered biosafe due to inactivating point mutations in both the env and vpr reading frames. There are 8 total spectra, 4 are from unlabeled aliquots of sample, and 4 are from aliquots of sample treated with glutathione to label dehydroamino acids.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Hiv-1 Vector Pnl4-3 (ncbitaxon:151458) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lloyd M. Smith

PROVIDER: MSV000088220 | MassIVE | Tue Oct 12 07:27:00 BST 2021

REPOSITORIES: MassIVE

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