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Proteomic shifts reflecting oxidative stress and reduced capacity for protein synthesis, and alterations to mitochondrial membranes in Neurospora crassa lacking VDAC

ABSTRACT: Proteomic analysis of N. crassa was performed to explore the role of VDAC (voltage dependent anion-selective channels) in the regulation of cellular processes. Differential label free quantitation was applied to both wild-type and VDAC-knockout versions, involving both cytosolic and mitochondrial isolations. Proteins involved in amino acid and protein synthesis were lowered by VDAC deletion, while stress responses and pyruvate metabolism were over-expressed. The absence of VDAC also impacted membrane lipids and hyphal morphology.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Neurospora Crassa (ncbitaxon:5141)

SUBMITTER: Deborah Court

PROVIDER: MSV000088567 | MassIVE | Tue Dec 14 11:51:00 GMT 2021

REPOSITORIES: MassIVE

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Proteomic Shifts Reflecting Oxidative Stress and Reduced Capacity for Protein Synthesis, and Alterations to Mitochondrial Membranes in Neurospora crassa Lacking VDAC.

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