Proteomics

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Pig Serum Proteome during Conventional and Extracorporeal Resuscitation


ABSTRACT: Pigs underwent 5 min of untreated cardiac arrest followed by 8 min of basic life support and 22 min of advanced life support (ALS). Following this conventional cardiopulmonary resuscitation, all pigs were treated 120 min with extracorporeal circulation (eCPR). Blood samples were drawn at baseline, after ALS and after eCPR and were processed to serum samples, which were depleted for Albumin and IgG before analysing them via explorative mass spectrometry.

INSTRUMENT(S): Q Exactive Plus, LTQ Orbitrap Elite

ORGANISM(S): Sus Scrofa (ncbitaxon:9823)

SUBMITTER: Prof. Oliver Schilling  

PROVIDER: MSV000088595 | MassIVE | Tue Dec 21 08:01:00 GMT 2021

REPOSITORIES: MassIVE

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Publications


<h4>Background</h4>Only a small number of patients survive an out-of-hospital cardiac arrest (CA) and can be discharged from hospital alive with a large percentage of these patients retaining neurological impairments. In recent years, extracorporeal cardiopulmonary resuscitation (ECPR) has emerged as a beneficial strategy to optimize cardiac arrest treatment. However, ECPR is still associated with various complications. To reduce these problems, a profound understanding of the underlying mechani  ...[more]

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