Dataset Information

Pig Serum Proteome during Conventional and Extracorporeal Resuscitation

ABSTRACT: Pigs underwent 5 min of untreated cardiac arrest followed by 8 min of basic life support and 22 min of advanced life support (ALS). Following this conventional cardiopulmonary resuscitation, all pigs were treated 120 min with extracorporeal circulation (eCPR). Blood samples were drawn at baseline, after ALS and after eCPR and were processed to serum samples, which were depleted for Albumin and IgG before analysing them via explorative mass spectrometry.

INSTRUMENT(S): Q Exactive Plus, LTQ Orbitrap Elite

ORGANISM(S): Sus Scrofa (ncbitaxon:9823)

SUBMITTER: Prof. Oliver Schilling

PROVIDER: MSV000088595 | MassIVE | Tue Dec 21 08:01:00 GMT 2021

REPOSITORIES: MassIVE

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Publications

Serum proteome alterations during conventional and extracorporeal resuscitation in pigs.

Bernhard Patrick P Bretthauer Berit Amelie BA Brixius Sam Joé SJ Bügener Hannah H Groh Johannes Elias JE Scherer Christian C Damjanovic Domagoj D Haberstroh Jörg J Trummer Georg G Benk Christoph C Beyersdorf Friedhelm F Schilling Oliver O Pooth Jan-Steffen JS

Journal of translational medicine 20220523 1

<h4>Background</h4>Only a small number of patients survive an out-of-hospital cardiac arrest (CA) and can be discharged from hospital alive with a large percentage of these patients retaining neurological impairments. In recent years, extracorporeal cardiopulmonary resuscitation (ECPR) has emerged as a beneficial strategy to optimize cardiac arrest treatment. However, ECPR is still associated with various complications. To reduce these problems, a profound understanding of the underlying mechani ...[more]

PMID: 35606879

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Project description:Background: Cerebral ischemia/reperfusion injury is a common secondary effect of cardiac arrest which is largely responsible for postresuscitative mortality. Therefore development of therapies which restore and protect the brain function after cardiac arrest is essential. Methylene blue (MB) has been experimentally proven neuroprotective in a porcine model of global ischemia-reperfusion in experimental cardiac arrest. However, no comprehensive analyses have been conducted at gene expression level. Methods: Pigs underwent either untreated cardiac arrest (CA) or CA with subsequent cardiopulmonary resuscitation (CPR) accompanied with an infusion of saline or an infusion of saline with MB. Genome-wide transcriptional profiling using the Affymetrix porcine microarray was performed to 1) gain understanding of delayed neuronal death initiation in porcine brain during ischemia and after 30, 60 and 180 min following reperfusion, and 2) identify the mechanisms behind the neuroprotective effect of MB after ischemic injury (at 30, 60 and 180 min). Results: Our results show that restoration of spontaneous circulation (ROSC) induces major transcriptional changes related to stress response, inflammation, apoptosis and even cytoprotection. In contrast, the untreated ischemic and anoxic insult affected only few genes mainly involved in intra-/extracellular ionic balance. Furthermore, our data show that the neuroprotective role of MB is diverse and fulfilled by regulation of the expression of soluble guanylate cyclase and biological processes accountable for inhibition of apoptosis, modulation of stress response, neurogenesis and neuroprotection. Conclusions: Our results support that MB could be a valuable intervention and should be investigated as a therapeutic agent against neural damage associated with I/R injury induced by cardiac arrest. One group of pigs underwent cardiac arrest (CA) without subsequent cardiopulmonary resuscitation (CPR) and the brain of the animals was removed after 5, 20 or 30 min post CA for genome-wide expression study. Two groups of pigs underwent first CA for 12 min with a subsequent 8 min-long CPR and received either an infusion of saline or an infusion of saline with MB from one minute after the start of CPR until 50 min after return of spontaneous circulation (ROSC). In both latter groups the brains were removed for microarray analysis at the following time points: 30, 60, 180 min.