Implantation-on-a-chip cellular proteome

ABSTRACT: We have designed an implantation-on-a-chip device to model the invasion of specialized fetal extravillous trophoblasts (EVTs) from an implanting embryo into the maternal decidua. We profiled the cellular proteome of sorted EVTs or endothelial cells from devices containing different cell compositions: 1) EVTs monoculture, 2) Endothelial cells monoculture, and 3) EVTs and endothelial cells co-cultured. The proteins were extracted using the MPLEx extraction method (Nakayasu et. al. PMCID: PMC5069757), digested with 1:50 (trypsin:protein, w:w) for 3 hr at 37 C. Solid phase extraction (SPE) was performed on the samples using 1 mL/50 mg columns from Phenomenex as previously described (PMCID: PMC7192326). Peptidic concentration was assayed using a BCA assay and 5 ul at 0.1 ug/ul were injected on the LC-MS/MS system. Data was searched with MaxQuant.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geremy Clair  

PROVIDER: MSV000088677 | MassIVE | Wed Jan 12 16:10:00 GMT 2022

REPOSITORIES: MassIVE