Project description:Native metabolomics method validation for chymotrypsin.
1. Limit of detection Mollasamide- Chymotrypsin
2. Flowinjection of Mollasamide over UHPLC gradients (with make-up).
3. Binding tests with chymotrypsin and different standards.
Project description:Native metabolomics method validation for chymotrypsin.
1. Limit of detection Mollasamide- Chymotrypsin
2. Flowinjection of Mollasamide over UHPLC gradients (with make-up).
3. Binding tests with chymotrypsin and different standards.
Project description:After screening some understudied Australian native fruits. The potential metabolites in the most active extracts (Water and ethanol extracts of Native currant) were analysed with liquid chromatography-mass spectrometry (LC-MS) driven metabolomics and chemometrics to spot differential and major metabolites. The metabolomics analysis revealed an abundance of flavonoids, fatty acyl derivatives, carbohydrates, carboxylic acids and their derivatives, and alkaloid compounds as potential bioactive metabolites in the NC extracts.
Project description:Native and non-targeted metabolomics with E.coli CutA and cell extracts of E.coli and Synechococcus elongatus PCC 7942. Cell extraction with 20% or 80% MeOH.
Project description:Mass developments of toxin-producing cyanobacteria are frequently observed in freshwater ecosystems due to eutrophication and global warming. These mass developments can partly be attributed to cyanobacterial toxins, such as protease inhibitors (PIs), which inhibit digestive serine proteases of Daphnia, the major herbivore of phytoplankton and cyanobacteria. To date, mechanisms of this inhibition in the gut of the crustacean Daphnia magna are not known. Here, we characterize a single serine protease, chymotrypsin 448 (CT448), which is present in the gut of the crustacean D. magna.