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Proteomic profiling of intra-islet features reveals substructure-specific protein signatures

ABSTRACT: LCM-coupled nanoPOTS proteomics using FAIMS of intra-islet microstructures. 5 acinar samples, 5 whole islets, 5 islet interface vasculature pools, 5 islet inner microvasculature pools, 3 islets after microsvasculature removed.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: Jon Jacobs

PROVIDER: MSV000088730 | MassIVE | Tue Jan 25 13:24:00 GMT 2022

REPOSITORIES: MassIVE

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Proteomic Profiling of Intra-Islet Features Reveals Substructure-Specific Protein Signatures.

Swensen Adam C AC Veličković Dušan D Williams Sarah M SM Moore Ronald J RJ Day Le Z LZ Niessen Sherry S Hennessy Sarah S Posso Camilo C Monetti Mara M Qian Wei-Jun WJ Jacobs Jon J Whiteley Laurence L Zhu Ying Y Piehowski Paul D PD

Molecular & cellular proteomics : MCP 20221014 12

Despite their diminutive size, islets of Langerhans play a large role in maintaining systemic energy balance in the body. New technologies have enabled us to go from studying the whole pancreas to isolated whole islets, to partial islet sections, and now to islet substructures isolated from within the islet. Using a microfluidic nanodroplet-based proteomics platform coupled with laser capture microdissection and field asymmetric waveform ion mobility spectrometry, we present an in-depth investig ...[more]

PMID: 36244662

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