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The ankyrin repeat protein RARP-1 is a periplasmic factor that supports Rickettsia parkeri growth and host cell invasion

ABSTRACT: Rickettsia spp. are obligate intracellular bacterial pathogens that have evolved a variety of strategies to exploit their host cell niche. However, the bacterial factors that contribute to this intracellular lifestyle are poorly understood. Here, we show that the conserved ankyrin repeat protein RARP-1 supports Rickettsia parkeri infection. Specifically, RARP-1 promotes efficient host cell entry and growth within the host cytoplasm, but is not necessary for cell-to-cell spread or evasion of host autophagy. We further demonstrate that RARP-1 is not secreted into the host cytoplasm by R. parkeri. Instead, RARP-1 resides in the periplasm, and we identify several binding partners that are predicted to work in concert with RARP-1 during infection. Altogether, our data reveal that RARP-1 plays a critical role in the rickettsial life cycle.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Rickettsia Parkeri Str. Portsmouth (ncbitaxon:1105108)

SUBMITTER: Rebecca Lamason

PROVIDER: MSV000088867 | MassIVE | Fri Feb 18 14:29:00 GMT 2022

REPOSITORIES: MassIVE

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