Proteome Alterations during Clonal Isolation of Human Pancreatic Cancer Cell Lines
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ABSTRACT: Starting from an initial cell culture (Reference), three human pancreatic cancer cell lines (MIA PaCa-2, AsPC-1 and PANC-1) were either maintained by standard cell culture (Standard) or underwent clonal isolation to obtain single-cell colonies (SCC). Emerging cell pellets of five replicates per condition were subjected to proteomic sample preparation with subsequent isobaric peptide labelling with tandem mass tags (TMTpro-16plex; leaving one channel empty). All samples of one cell line were pooled and subjected to high-pH fractionation, resulting in 12 fractions which were subsequently analyzed via explorative mass spectrometry.
INSTRUMENT(S): Q Exactive Plus
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Prof. Oliver Schilling
PROVIDER: MSV000088904 | MassIVE | Wed Feb 23 11:53:00 GMT 2022
REPOSITORIES: MassIVE
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