Ammonium Sulfate-based Prefractionation Improved Proteome Coverage in Arabidopsis thaliana Leaf Extract

ABSTRACT: The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed, of which the results validated this hypothesis. Of note, this prefractionation method allowed to identify 45% more proteins compared to the number of proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry drastically improved the detection of intact and post-translationally modified proteins.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Tagnon Missihoun  

PROVIDER: MSV000088920 | MassIVE | Fri Feb 25 11:58:00 GMT 2022

SECONDARY ACCESSION(S): PXD031898

REPOSITORIES: MassIVE