ERG-Proximity biotin labeling_2022-03-30
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Brian Raught
PROVIDER: MSV000089158 | MassIVE | Wed Mar 30 06:27:00 BST 2022
SECONDARY ACCESSION(S): PXD032874
REPOSITORIES: MassIVE
Similar Datasets
Project description:We performed a BioID experiment in a HEK293 Flp-In T-Rex cell line that contains human GNL3 cDNA integrated at the FRT site tagged with BirA and FLAG under the control of a promoter regulated with doxycycline.
2023-12-05 | PXD045389 | Pride
Project description:BioID Controls for Flp-In T-REx HEK293 cells are the following;
Empty Vector:
VL_20151116_COT_01_HEK293_pcDNA5EV_HB
VL_20151116_COT_02_HEK293_pcDNA5EV_HB
VL_20151116_COT_03_HEK293_pcDNA5EV_HB
VL_20151116_COT_04_HEK293_pcDNA5EV_HB
BirA-Flag-EGFP:
VL_20150825_COT_05_HB
VL_20150825_COT_06_HB
VL_20150825_COT_07_HB
VL_20150825_COT_08_HB
BirA-Flag-EGFP-CAAX :
VL_20181019_COT_01_HB
VL_20181019_COT_02_HB
VL_20181019_COT_03_HB
VL_20181019_COT_04_HB
BioID samples for Flp-In T-REx HEK293 cells are the following;
RAC1_WT:
2186_ZL_RAC1_WT_BR1_Velos1_P103_HB
2308_ZL_RAC1_WT_BR2_Velos1_HB
RHOG_WT:
VL_20170123_COT_01_HB
VL_20170123_COT_02_HB
RHOA_WT:
VL_20160323_COT_04new2_HB
VL_20180104_COT_01_HB
CDC42_WT:
VL_20180104_COT_03_HB
VL_20180104_COT_04_HB
RAC1_G15A:
VL_20180105_COT_01_2_HB
VL_20180105_COT_02_HB
CDC42_G15A:
VL_20180105_COT_03_HB
VL_20180105_COT_04_HB
RHOG_G15A:
VL_20180108_COT_01_HB
VL_20180108_COT_02_HB
RHOA_G17A:
VL_20180108_COT_03_HB
VL_20180108_COT_04_HB
CDC42_G12V:
2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB
2263_ZL_CDC42_G12V_BR2_Velos1_HB
RAC1_G12V:
1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB
VL_20150825_COT_02_HB
RAC2_G12V:
2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB
2323_ZL_RAC2_G12V_BR2_Velos1_HB
RAC3_G12V:
2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB
2321_ZL_RAC3_G12V_BR2_Velos1_HB
RHOA_G14V:
2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB
2262_ZL_RHOA_G14V_BR2_Velos1_HB
RHOB_G14V:
2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB
2261_ZL_RHOB_G14V_BR2_Velos1_HB
RHOBTB1_WT:
2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB
2320_ZL_RHOBTB1_WT_BR2_Velos1_HB
RHOBTB2_WT:
2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB
2318_ZL_RHOBTB2_WT_BR2_Velos1_HB
RHOC_G14V:
2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB
2260_ZL_RHOC_G14V_BR2_Velos1_HB
RHOD_G26V:
2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB
2259_ZL_RHOD_G26V_BR2_Velos1_HB
RHOF_G28V:
2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB
2258_ZL_RHOF_G28V_BR2_Velos1_HB
RHOG_G12V:
2306_ZL_RHOG_G12V_BR2_Velos1_HB
VL_20170123_COT_03_HB
RHOH_WT:
2203_ZL_RHOH_WT_BR1_Velos1_P103_HB
2317_ZL_RHOH_WT_BR2_Velos1_HB
RHOJ_G40V:
2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB
2257_ZL_RHOJ_G40V_BR2_Velos1_HB
RHOQ_G18V:
2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB
2338_ZL_RHOQ_G18V_BR2_Velos1_HB
RHOU_G58V:
2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB
2337_ZL_RHOU_G58V_BR2_Velos1_HB
RHOV_G40V:
2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB
2324_ZL_RHOV_G40V_BR2_Velos1_HB
RND1_WT:
2249_ZL_RND1_WT_BR1_Velos1_P103_HB
2313_ZL_RND1_WT_BR2_Velos1_HB
RND2_WT:
2247_ZL_RND2_WT_BR1_Velos1_P103_HB
2315_ZL_RND2_WT_BR2_Velos1_HB
RND3_WT:
VL_20151116_COT_05_HB
VL_20151116_COT_06_HB
BioID Controls for Flp-In T-REx HeLa cells are the following;
Empty Vector:
QE_20180917_COT_01_HB
QE_20180917_COT_02_HB
QE_20180917_COT_03_HB
QE_20180917_COT_04_HB
BirA-Flag-EGFP:
QE_20151030_COT_02_HB
QE_20151030_COT_03_HB
QE_20151030_COT_04_HB
QE_20151030_COT_05_HB
BirA-Flag-EGFP-CAAX:
QE_20181022_COT_01_HB
QE_20181022_COT_02_HB
QE_20181022_COT_03_HB
QE_20181022_COT_04_HB
BioID samples for Flp-In T-REx HeLa cells are the following;
CDC42_G15A:
QE_20171218_COT_05_HB
QE_20171218_COT_06_HB
CDC42_WT:
QE_20171218_COT_01_HB
QE_20171218_COT_02_HB
RAC1_G15A:
QE_20171218_COT_03_HB
QE_20171218_COT_04_HB
RAC1_WT:
QE_20151102_COT_01_HB
QE_20151102_COT_02_HB
RHOA_G17A:
QE_20171218_COT_07_HB
QE_20171218_COT_08_HB
RHOA_WT:
QE_20171201_COT_07_HB
QE_20171201_COT_08_HB
RHOG_G15A:
QE_20171220_COT_01_HB
QE_20171220_COT_02_HB
RHOG_WT:
QE_20161215_COT_01_HB
QE_20161215_COT_02_HB
CDC42_G12V:
QE_20171201_COT_09_HB
QE_20171201_COT_10_HB
RAC1_G12V:
QE_20151030_COT_06_HB
QE_20151030_COT_07_HB
RAC2_G12V:
QE_20180105_COT_05_HB
QE_20180105_COT_06_HB
RAC3_G12V:
QE_20180105_COT_07_HB
QE_20180105_COT_08_HB
RHOA_G14V:
QE_20171205_COT_17_HB
QE_20171205_COT_18_HB
RHOB_G14V:
QE_20180105_COT_01_HB
QE_20180105_COT_02_HB
RHOBTB1_WT:
QE_20180108_COT_07_HB
QE_20180108_COT_08_HB
RHOBTB2_WT:
QE_20171205_COT_19_HB
QE_20171205_COT_20_HB
RHOC_G14V:
QE_20180105_COT_03_HB
QE_20180105_COT_04_HB
RHOD_G26V:
QE_20180108_COT_05_HB
QE_20180108_COT_06_HB
RHOF_G28V:
QE_20171130_COT_05_HB
QE_20171130_COT_06_HB
RHOG_G12V:
QE_20161215_COT_03_HB
QE_20161215_COT_04_HB
RHOH_WT:
QE_20171204_COT_15_HB
QE_20171204_COT_16_HB
RHOJ_G40V:
QE_20171130_COT_03_HB
QE_20171130_COT_04_HB
RHOQ_G18V:
QE_20180105_COT_09_HB
QE_20180105_COT_10_HB
RHOU_G58V:
QE_20171204_COT_11_HB
QE_20171204_COT_12_new_HB
RHOV_G40V:
QE_20171130_COT_01_HB
QE_20171130_COT_02_HB
RND1_WT:
QE_20180108_COT_01_HB
QE_20180108_COT_02_HB
RND2_WT:
QE_20171204_COT_13_HB
QE_20171204_COT_14_HB
RND3_WT:
QE_20180108_COT_03_HB
QE_20180108_COT_04_HB
2019-10-20 | MSV000084477 | MassIVE
Project description:BioID Controls for Flp-In T-REx HEK293 cells are the following;
Empty Vector:
VL_20151116_COT_01_HEK293_pcDNA5EV_HB
VL_20151116_COT_02_HEK293_pcDNA5EV_HB
VL_20151116_COT_03_HEK293_pcDNA5EV_HB
VL_20151116_COT_04_HEK293_pcDNA5EV_HB
BirA-Flag-EGFP:
VL_20150825_COT_05_HB
VL_20150825_COT_06_HB
VL_20150825_COT_07_HB
VL_20150825_COT_08_HB
BirA-Flag-EGFP-CAAX:
VL_20181019_COT_01_HB
VL_20181019_COT_02_HB
VL_20181019_COT_03_HB
VL_20181019_COT_04_HB
BioID samples for Flp-In T-REx HEK293 cells are the following;
RAC1_WT:
2186_ZL_RAC1_WT_BR1_Velos1_P103_HB
2308_ZL_RAC1_WT_BR2_Velos1_HB
RHOG_WT:
VL_20170123_COT_01_HB
VL_20170123_COT_02_HB
RHOA_WT:
VL_20160323_COT_04new2_HB
VL_20180104_COT_01_HB
CDC42_WT:
VL_20180104_COT_03_HB
VL_20180104_COT_04_HB
RAC1_G15A:
VL_20180105_COT_01_2_HB
VL_20180105_COT_02_HB
CDC42_G15A:
VL_20180105_COT_03_HB
VL_20180105_COT_04_HB
RHOG_G15A:
VL_20180108_COT_01_HB
VL_20180108_COT_02_HB
RHOA_G17A:
VL_20180108_COT_03_HB
VL_20180108_COT_04_HB
CDC42_G12V:
2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB
2263_ZL_CDC42_G12V_BR2_Velos1_HB
RAC1_G12V:
1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB
VL_20150825_COT_02_HB
RAC2_G12V:
2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB
2323_ZL_RAC2_G12V_BR2_Velos1_HB
RAC3_G12V:
2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB
2321_ZL_RAC3_G12V_BR2_Velos1_HB
RHOA_G14V:
2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB
2262_ZL_RHOA_G14V_BR2_Velos1_HB
RHOB_G14V:
2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB
2261_ZL_RHOB_G14V_BR2_Velos1_HB
RHOBTB1_WT:
2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB
2320_ZL_RHOBTB1_WT_BR2_Velos1_HB
RHOBTB2_WT:
2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB
2318_ZL_RHOBTB2_WT_BR2_Velos1_HB
RHOC_G14V:
2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB
2260_ZL_RHOC_G14V_BR2_Velos1_HB
RHOD_G26V:
2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB
2259_ZL_RHOD_G26V_BR2_Velos1_HB
RHOF_G28V:
2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB
2258_ZL_RHOF_G28V_BR2_Velos1_HB
RHOG_G12V:
2306_ZL_RHOG_G12V_BR2_Velos1_HB
VL_20170123_COT_03_HB
RHOH_WT:
2203_ZL_RHOH_WT_BR1_Velos1_P103_HB
2317_ZL_RHOH_WT_BR2_Velos1_HB
RHOJ_G40V:
2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB
2257_ZL_RHOJ_G40V_BR2_Velos1_HB
RHOQ_G18V:
2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB
2338_ZL_RHOQ_G18V_BR2_Velos1_HB
RHOU_G58V:
2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB
2337_ZL_RHOU_G58V_BR2_Velos1_HB
RHOV_G40V:
2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB
2324_ZL_RHOV_G40V_BR2_Velos1_HB
RND1_WT:
2249_ZL_RND1_WT_BR1_Velos1_P103_HB
2313_ZL_RND1_WT_BR2_Velos1_HB
RND2_WT:
2247_ZL_RND2_WT_BR1_Velos1_P103_HB
2315_ZL_RND2_WT_BR2_Velos1_HB
RND3_WT:
VL_20151116_COT_05_HB
VL_20151116_COT_06_HB
BioID Controls for Flp-In T-REx HeLa cells are the following;
Empty Vector:
QE_20180917_COT_01_HB
QE_20180917_COT_02_HB
QE_20180917_COT_03_HB
QE_20180917_COT_04_HB
BirA-Flag-EGFP:
QE_20151030_COT_02_HB
QE_20151030_COT_03_HB
QE_20151030_COT_04_HB
QE_20151030_COT_05_HB
BirA-Flag-EGFP-CAAX:
QE_20181022_COT_01_HB
QE_20181022_COT_02_HB
QE_20181022_COT_03_HB
QE_20181022_COT_04_HB
BioID samples for Flp-In T-REx HeLa cells are the following;
CDC42_G15A:
QE_20171218_COT_05_HB
QE_20171218_COT_06_HB
CDC42_WT:
QE_20171218_COT_01_HB
QE_20171218_COT_02_HB
RAC1_G15A:
QE_20171218_COT_03_HB
QE_20171218_COT_04_HB
RAC1_WT:
QE_20151102_COT_01_HB
QE_20151102_COT_02_HB
RHOA_G17A:
QE_20171218_COT_07_HB
QE_20171218_COT_08_HB
RHOA_WT:
QE_20171201_COT_07_HB
QE_20171201_COT_08_HB
RHOG_G15A:
QE_20171220_COT_01_HB
QE_20171220_COT_02_HB
RHOG_WT:
QE_20161215_COT_01_HB
QE_20161215_COT_02_HB
CDC42_G12V:
QE_20171201_COT_09_HB
QE_20171201_COT_10_HB
RAC1_G12V:
QE_20151030_COT_06_HB
QE_20151030_COT_07_HB
RAC2_G12V:
QE_20180105_COT_05_HB
QE_20180105_COT_06_HB
RAC3_G12V:
QE_20180105_COT_07_HB
QE_20180105_COT_08_HB
RHOA_G14V:
QE_20171205_COT_17_HB
QE_20171205_COT_18_HB
RHOB_G14V:
QE_20180105_COT_01_HB
QE_20180105_COT_02_HB
RHOBTB1_WT:
QE_20180108_COT_07_HB
QE_20180108_COT_08_HB
RHOBTB2_WT:
QE_20171205_COT_19_HB
QE_20171205_COT_20_HB
RHOC_G14V:
QE_20180105_COT_03_HB
QE_20180105_COT_04_HB
RHOD_G26V:
QE_20180108_COT_05_HB
QE_20180108_COT_06_HB
RHOF_G28V:
QE_20171130_COT_05_HB
QE_20171130_COT_06_HB
RHOG_G12V:
QE_20161215_COT_03_HB
QE_20161215_COT_04_HB
RHOH_WT:
QE_20171204_COT_15_HB
QE_20171204_COT_16_HB
RHOJ_G40V:
QE_20171130_COT_03_HB
QE_20171130_COT_04_HB
RHOQ_G18V:
QE_20180105_COT_09_HB
QE_20180105_COT_10_HB
RHOU_G58V:
QE_20171204_COT_11_HB
QE_20171204_COT_12_new_HB
RHOV_G40V:
QE_20171130_COT_01_HB
QE_20171130_COT_02_HB
RND1_WT:
QE_20180108_COT_01_HB
QE_20180108_COT_02_HB
RND2_WT:
QE_20171204_COT_13_HB
QE_20171204_COT_14_HB
RND3_WT:
QE_20180108_COT_03_HB
QE_20180108_COT_04_HB
2019-03-21 | MSV000083608 | MassIVE
Project description:To gain insights into the interactome of wild-type (WT) and S102P mutant GATAD1, we utilized the BioID method, which enables the study of protein-protein interactions. Specifically, we performed BioID proximity labeling experiments in stable Flp-In cells expressing different GATAD1 variants fused to BirA*_FLAG. These variants included BirA*_FLAG_GATAD1-WT, BirA*_FLAG_GATAD1-S102P, BirA*_FLAG_GATAD1-S102D, and BirA*_FLAG_GATAD1-S102A. By employing this approach, we aimed to characterize the protein interactors associated with these GATAD1 variants and gain insights into the functional consequences of the S102P mutation.
2024-02-15 | PXD043789 | Pride
Project description:Data contains LC-MS results of proximity-dependent biotin labeling to generate the protein interaction network of IRS1 protein fused with Flag-miniTurbo (N-terminal tag)
and expressed in T-REx HEK293 cells.
| MSV000088166 | MassIVE
Project description:triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.
2015-09-16 | PXD002127 | Pride
Project description:ChIP-seq analyses were performed in MEL cells expressing BirA alone or BirA and FLAG-Biotin tagged BCL11A (XL isoform).
2014-04-01 | GSE28334 | GEO
Project description:RNAi-seq of stable Flp-In™ T-Rex Hela cells that inducibly expressed PP1-NIPP1 and mutants.
2018-06-28 | GSE104320 | GEO
Project description:UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction activates NMD and leads to the degradation of the mRNA. To investigate the role of UPF3B in NMD, we have generated FLAG-TurboID-UPF3B wild type or mutant expressing human Flp-In T-Rex 293 UPF3B knockout cells using the PiggyBac transposon system. We generated proteomic data from the proximity labeled interactome of these UPF3B variants.
2022-05-04 | PXD032337 | Pride
Project description:UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction activates NMD and leads to the degradation of the mRNA. To investigate the role of UPF3B in NMD, we have generated FLAG-TurboID-UPF3B wild type or mutant expressing human Flp-In T-Rex 293 UPF3B knockout cells using the PiggyBac transposon system. We generated proteomic data from the proximity labeled interactome of these UPF3B variants.
2022-05-04 | PXD029898 | Pride