Dataset Information

Human HEK293T and Pig cardiac top-down proteomes from Bruker maXis II

ABSTRACT: These data, derived from PXD019368 (PMID 33231430) and PXD010825 (PMID 30988469) represent a test of deconvolution algorithms for top-down proteomics experiments on the Bruker maXis II Q-TOF. Three LC-MS/MS experiments are available from pig cardiac tissue in both raw .d directories and in deconvolved msAlign files produced by Bruker DataAnalysis 5.3, TopFD 1.4.13, FLASHDeconv 2.0, and Mascot Distiller 2.8. Eight LC-MS/MS experiments are available from human HEK293T cell lysates, with four produced using Triton and four produced using Tergitol. The inferred precursor charges, precursor masses, and numbers of tandem mass spectra diverge considerably depending upon deconvolution algorithm.

INSTRUMENT(S): maXis II

ORGANISM(S): Sus Scrofa (ncbitaxon:9823) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ying Ge

PROVIDER: MSV000089252 | MassIVE | Wed Apr 13 09:43:00 BST 2022

SECONDARY ACCESSION(S): PXD033208

REPOSITORIES: MassIVE

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Publications

Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry.

Brown Kyle A KA Tucholski Trisha T Alpert Andrew J AJ Eken Christian C Wesemann Lucas L Kyrvasilis Andreas A Jin Song S Ge Ying Y

Analytical chemistry 20201124 24

Although top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems, the analysis of endogenous membrane proteins remains challenging due to their low solubility, low abundance, and the complexity of the membrane subproteome. Here, we report a simple but effective enrichment and separation strategy for top-down proteomics of endogenous membrane proteins enabled by cloud point extraction and multidimensional liquid chromatography coupled to high-resoluti ...[more]

PMID: 33231430

Publication: 1/2

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