Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome asymmetry in mouse and human embryos before fate specification

ABSTRACT: Raw files of early-stage mouse and human blastomeres & mouse zygotes

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Nikolai Slavov

PROVIDER: MSV000089353 | MassIVE | Mon May 02 08:39:00 BST 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Fertilization triggers early proteomic symmetry breaking in mammalian embryos.

Iwamoto-Stohl Lisa K LK Petelski Aleksandra A AA Quan Baiyi B Meglicki Maciej M Fu Audrey A Nakagawa Shoma S McMahon Breanna B Wang Ting-Yu TY Khan Saad S Specht Harrison H Huffman Gray G Derks Jason J Junyent Sergi S Weatherbee Bailey A T BAT Weberling Antonia A Gantner Carlos W CW Mandelbaum Rachel S RS Paulson Richard J RJ Lam Lisa L Chou Tsui-Fen TF Slavov Nikolai N Zernicka-Goetz Magdalena M

Cell 20251203

While non-mammalian embryos often rely on spatial pre-patterning, mammalian development has long been thought to begin with equivalent blastomeres. However, emerging evidence challenges this. Here, using multiplexed and label-free single-cell proteomics, we identify over 300 asymmetrically abundant proteins-many involved in protein degradation and transport-dividing mouse 2-cell-stage blastomeres into two distinct clusters, which we term alpha and beta. These proteomic asymmetries are detectable ...[more]

PMID: 41344326

Similar Datasets

Proteome analysis of mouse Wdr74-knockout embryos

Project description:Mouse Wdr74-knockout embryos were generated using the CRISPR-Cas9 system. Embryos from the 4-cell stage to the morula stage were collected and treated with acidic Tyrode's solution to remove the zona pellucida. We used 50 embryos per sample at each developmental stage and identified the proteome of mouse Wdr74-knockout embryos by label-free quantification.

2025-05-22 | PXD059124 | JPOST Repository

Proteome analysis of mouse oocytes and pre-implantation embryos

Project description:Mouse oocytes and embryos from the germinal vesicle (GV) stage to the blastocyst stage were collected and treated with acidic Tyrode’s solution to remove the zona pellucida. We used 42.5 oocytes or embryos per sample at each developmental stage and identified the proteome during mouse pre-implantation development by label-free quantification.

2025-05-22 | PXD059122 | JPOST Repository

Identification of endoderm-specific genes in mouse embryos

Project description:The foregut definitive endoderm is the precursor of many tissues including the liver, pancreas, thyroid, lungs, trachea and oesophagus. However, networks and pathways involved in the early development of the definitive endoderm of mammals are not well studied. To identify genes with potential roles in the early development of the foregut definitive endoderm in mouse embryos, we performed microarray analysis to compare the gene expression profile of foregut endoderm and non-endodermal tissues from early somite-stage mouse embryos.

2012-03-13 | GSE36453 | GEO

Gene expression changes in GADD45 deficient 2-cell mouse embryos

Project description:To study the role of GADD45 proteins in early mouse development, RNA-seq expression profiling was performed with Gadd45a/Gadd45b double knockout (DKO) and wild-type (WT) mouse embryos at 2-cell stage (2C).

2019-05-31 | GSE127719 | GEO

Single cell analysis for oocytes and early embryos

Project description:While non-mammalian embryos often rely on spatial pre-patterning, for decades it was believed that blastomeres of mouse and human embryos were developmentally equivalent. However, emerging evidence suggests early mouse and human blastomeres differ in developmental potential. Here, using multiplexed and label-free single-cell proteomics, we identify over three hundred asymmetrically abundant proteins - many involved in protein degradation and transport - that divide mouse 2-cell stage blastomeres into two distinct clusters, which we term alpha and beta. These asymmetries intensify at the 4-cell stage and are detectable as early as the zygote. Fertilisation acts as a trigger for this breaking of symmetry and sperm entry point correlates with the gradient of proteome asymmetry. Perturbation of beta-enriched proteins (Gps1, Nedd8) alters lineage segregation. Embryos derived from beta blastomeres have more epiblast cells, while alpha blastomeres are associated with a lower developmental potential. Human 2-cell embryos show similar clustering and enrichment patterns, supporting conservation of this early asymmetry. Our findings reveal a previously unrecognized proteomic pre-patterning triggered by fertilisation in mammalian embryos, with implications for understanding totipotency and early lineage bias.

2025-08-10 | MSV000098778 | MassIVE

Comparative maternal protein profiling of mouse biparental and uniparental embryos

Project description:During oogenesis, RNAs and proteins from maternal genome will be accumulated and they are the most important regulatory factors in early embryonic development. Parthenogenesis provides a unique source to investigate the influence of maternal genomes in early mammalian development and is proposed as an experimental tool to investigate embryo development which may solve many of the ethical concerns. Using label-free quantitative mass sepctrometry (MS), we systematically monitored protein expression profiles from six stages during pre-implantation development used by parthenogenesis model of mouse: pronucleus-, 2-cell, 4-cell, and 8-cell embryo, morula, and blastocyst. They are labeled as PA, PA-2, PA-4, PA-8, PAMO, PABL, respectively. For each stage, 6,000 embryos were used, and the experiment was performed in three biological replicates, and they had 2,048 proteins in common.

2022-07-15 | PXD029532 |

Mouse gut metagenomes

Project description:Mouse gut metagenomes at the early stage after weaning

| PRJEB50255 | ENA

Transcription inhibition in early mouse embryos impacts transcriptome at 4-cell stage

Project description:Mouse zygotes were treated with DRB to inhibit transcription activity, and early embryos were collected at 4-cell stage to examine transcriptome alteration by RNA-seq.

2025-01-20 | GSE282667 | GEO

Fertilization triggers a transcription program mouse & human one-cell embryos

Project description:Following fertilization, the new embryo reprograms parental genomes to begin transcription (embryonic genome activation, EGA). EGA is indispensable for development, but its dynamics, profile or when it initiates in vertebrates are unknown. We here characterize the onset of transcription in mouse one-cell embryos. Precise embryo staging eliminated noise to reveal a cascading program of de novo transcription initiating within six hours of fertilization. This immediate EGA (iEGA) utilized canonical promoters, produced spliced transcripts, was distinctive and predominantly driven by the maternal genome. Expression represented pathways not only associated with embryo development but with cancer. In human one-cell embryos, hundreds of genes were up-regulated, days earlier than thought, with conservation to mouse iEGA. These findings provide a functional basis for epigenetic analysis in early-stage embryos and illuminate networks governing totipotency and other cell-fate transitions.

2021-12-22 | GSE157834 | GEO

Neogenin as a receptor for early cell fate determination in preimplantation mouse embryos.

Project description:The first cell lineage determination in embryos takes place when two cell populations are set apart, each differentiating into the trophectoderm (TE) and inner cell mass (ICM), respectively. It is widely believed that position/polarity cues play a key role in triggering this differentiation, but it remains unclear how extracellular cues are transduced into cell fate determination. Here, we provide evidence that supports that neogenin is implicated in relaying extracellular cues into the first cell fate determination in preimplantation mouse embryos. A polarized and transient distribution of neogenin was manifested in early blastomeres. Neogenin up-regulation by its overexpression accelerated ICM development in the blastocyst concomitant with the activation of the ICM-specific transcription factors Oct3/4, Sox2, and Nanog while its depletion by small hairpin RNAs (shRNAs) caused a developmental abnormality of poorly endowed ICM accompanied by the deactivation of Oct3/4, Sox2, and Nanog. Treatment with netrin-1 among neogenin ligands further impaired both embryonic development and ICM formation while repulsive guidance molecule c (RGMc) led to opposite consequences, enhancing ICM formation. From this study, we propose a model whereby neogenin interprets its own expression level to control the first cell fate determination in response to extracellular cues.

| S-EPMC4094428 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data