Oxonium ion scanning mass spectrometry for large-scale plasma glycoproteomics

ABSTRACT: Protein glycosylation, a complex and heterogeneous post-translational modification that is frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a data-independent acquisition technique for the large-scale mass-spectrometric quantification of glycopeptides in plasma samples. The technique, which we named ‘OxoScan-MS’, identifies oxonium ions as glycopeptide fragments and exploits a sliding-quadrupole dimension to generate comprehensive and untargeted oxonium- ion maps of precursor masses assigned to fragment ions from non-enriched plasma samples. By applying OxoScan-MS to quantify 1,002 glycopeptide features in the plasma glycoproteomes from patients with COVID-19 and healthy controls, we found that severe COVID-19 induces differential glycosylation in IgA, haptoglobin, transferrin and other disease-relevant plasma glycoproteins. OxoScan-MS may allow for the quantitative mapping of glycoproteomes at the scale of hundreds to thousands of samples.

INSTRUMENT(S): Orbitrap Eclipse, TripleTOF 6600, ZenoTOF 7600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prof. Dr. Markus Ralser  

PROVIDER: MSV000089567 | MassIVE | Sun May 29 10:57:00 BST 2022

SECONDARY ACCESSION(S): PXD034172

REPOSITORIES: MassIVE