Thermal proteome profiling of EliF fluorescent probe

ABSTRACT: In order to investigate cellular target proteins of EliF, a label-free target identification using thermal proteome profiling (TPP) was conducted. Briefly, Hela cells were incubated with EliF-1b or EliF-2c, and each sample was divided into 10 fractions (high pH fractionation protocol). A heat shock (ranges from 37C to 67C) was applied to each fraction to induce thermal denaturing. Non-denatured proteins were subsequently recovered, digested into peptides, and labeled with 10-plex TMT.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jun-Seok Lee  

PROVIDER: MSV000089586 | MassIVE | Wed Jun 01 22:07:00 BST 2022

REPOSITORIES: MassIVE