Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803

ABSTRACT: The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This protocol describes the acquisition of intact and active ATPase with good purity from Synechocystis sp. PCC 6803, based on a 3xFLAG tag fused to the beta subunit. The reproducibility of this protocol is high.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Synechocystis Sp. Pcc 6803 (ncbitaxon:1148)

SUBMITTER: Wolfgang R. Hess  

PROVIDER: MSV000089593 | MassIVE | Fri Jun 03 03:14:00 BST 2022

SECONDARY ACCESSION(S): PXD034273

REPOSITORIES: MassIVE