Development of an online 2D high-pH and low-pH reversed-phase nanoLC-MS/MS system for comprehensive proteome analysis

ABSTRACT: In bottom-up proteomics, the complexity of proteome profiling is usually limited by the number of identified proteins in nanoflow LC MS/MS analysis. Herein, we developed a fully automatic online 2D nanoLC MS/MS system using both high-pH and low-pH RPLCs for a comprehensive proteomics analysis.

INSTRUMENT(S): maXis, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Chao-Jung Chen  

PROVIDER: MSV000089609 | MassIVE | Sun Jun 05 22:29:00 BST 2022

REPOSITORIES: MassIVE