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Thermal proteome profiling reveals distinct target selectivity for differentially oxidized oxysterols

ABSTRACT: The dataset contains mass spectrometric raw data from Thermal Proteomic Profiling experiments aimed to identify the protein targets of three A- and B-ring oxidized sterols (cholestane-3beta,5alfa,6beta-triol (CT), 7keto-cholesterol (7-KC), 4beta-hydroxycholesterol (4beta-HC) ) and a side-chain oxidized sterol (25-hydroxycholesterol (25-HC)). The dataset comprises the raw data for the 4 different oxysterols, measured on both oxysterol (compound) and DMSO (vehicle), each collected in 28 fractions. The experiments were performed in three independent replicates for each oxysterol (R1-R3).

INSTRUMENT(S): Q-Exactive HF-X (Thermo Fisher)

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: CECILIA ROSSETTI

PROVIDER: MSV000089661 | MassIVE | Thu Jun 16 00:53:00 BST 2022

REPOSITORIES: MassIVE

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Thermal Proteome Profiling Reveals Distinct Target Selectivity for Differentially Oxidized Oxysterols.

Rossetti Cecilia C Laraia Luca L

ACS chemical biology 20220628 7

Oxysterols are produced physiologically by many species; however, their distinct roles in regulating human physiology have not been studied systematically. The role of differing oxidation states and sites in mediating their biological functions is also unclear. As oxysterols have been associated with atherosclerosis, neurodegeneration, and cancer, a better understanding of their protein targets is desirable. To address this, we mapped the oxysterol interactome with three A- and B-ring oxidized s ...[more]

PMID: 35763711

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