Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer

ABSTRACT: Proteomics analysis of purified lysosomes isolated from KP4 human pancreatic ductal adenocarcinoma cells grown in 2D treated with DMSO (CTRL) or Trametinib (Tram) for 48hrs in full growth media. Elutes were quantified for total protein and equal protein amounts were submitted for mass spectrometry. See Ravichandran et al Cancer Discovery 2022

INSTRUMENT(S): ThermoFisher Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Rushika M. Perera

PROVIDER: MSV000089676 | MassIVE | Thu Jun 16 19:43:00 BST 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [ChIP-Seq]

Project description:In this study, human pancreatic ductal adenocarcinoma cells lines (KP4, MiaPaca2) were treated with the MEK inhibitor, Trametinib or DMSO for 48hrs prior to RNA-seq profiling to evaluate significant gene expression changes associated with MEK inhibition. To assess the role of transcription factors MYC and TFE3 in regulation of gene expression changes associated with MEK inhibition, we performed chromatin immunoprecipitation using antibodies against c-MYC and TFE3 in KP4 cells following 48hrs of Trametinib or DMSO treatment.

2022-07-06 | GSE206268 | GEO

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [RNA-Seq]

2022-07-06 | GSE206267 | GEO

Cordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer_2

Project description:Whole cell proteomics of human pancreatic ductal adenocarcinoma cells - KP4 - treated for 48hrs with DMSO or 100nM Trametinib. Cell pellets were collected from n=3 biological replicates per condition. The samples were labeled with TMT reagents and combined together, fractionated into 96 fractions, and then pooled back into 24 fractions. 12 of the fractions were run and correspond to the 12 uploaded files.

2022-06-27 | MSV000089741 | MassIVE

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-06 | GSE206269 | GEO

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer

Project description:Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer

| PRJNA849928 | ENA

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [ChIP-Seq]

Project description:Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [ChIP-Seq]

| PRJNA849933 | ENA

Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [RNA-Seq]

Project description:Coordinated transcriptional and catabolic programs support iron dependent adaptation to RAS-MAPK pathway inhibition in pancreatic cancer [RNA-Seq]

| PRJNA849935 | ENA

DOCK8 is a Novel Regulator of Lysosome-Mediated Pancreatic Cancer Invasion

Project description:We found that oncogenic KRAS significantly enriches levels of the guanine nucleotide exchange factor (GEF) DOCK8 on lysosomes. DOCK8 is aberrantly expressed in a subset of Pancreatic Ductal Adenocarcinoma where it promotes cell invasion in vitro and in vivo.

2023-08-03 | GSE185335 | GEO

Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth

Project description:Lysosomes were immuno-isolated from HEK293T cells and PaTu8988T pancreatic ductal adenocarcinoma cells and analyzed by mass spectrometry based proteomics. Three independent biological replicates for each cell line is provided.

2021-01-26 | MSV000086769 | MassIVE